TABLE 1.

Kinetic parameters of the wild-type and HCE-Gly52_R ATCase^a

	pH	Vmaxb	[Asp]0.5	nHc
Wild-type	8.3	16.6 ± 0.4	12.6 ± 0.1	3.1 ± 0.4
	7.0	10.4 ± 0.1	7.5 ± 0.2	2.1 ± 0.2
HCE-Gly52R	8.3	16.8 ± 2.2	12.6 ± 0.7	2.7 ± 0.3
	7.0	8.7 ± 0.3	7.7 ± 0.5	1.7 ± 0.3

^aAll experiments were performed at 25 °C in 50 mM Tris-acetate buffer (pH 8.3) or 20 mM Bis-Tris, 20 mM Tris, 20 mM CAPS buffer (pH 7.0) at a saturating CP concentration (4.8 mM). The values reported are the average deviation of four determinations.

^bThe maximal observed velocity (mmol h⁻¹ mg⁻¹)

^cHill coefficient