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The pI was calculated using pK values derived from the report of Sillero Maldonado (2006 ▶). The ionic strengths of the reservoir solution were calculated using pKa values of acetate, HEPES and Tris buffers as 4.80, 7.55 and 8.06, respectively, and the lowest NaCl concentration in each pH when crystals are observed. The charge density was calculated using equation (1) with V M values shown in the table.
