FIG. 2.

H₂O₂- and UV-induced VDAC oligomerization. (A and B) T-REx-293 cells were incubated for 5 h with 1 mM H₂O₂, harvested, and subjected either to cross-linking with EGS (250 μM) as for Fig. 1 (A) or to apoptosis analysis via PI uptake and FACS analysis (B). The results of one experiment representative of three similar experiments are shown. (C) Cells were exposed to UV irradiation for the indicated times and were analyzed after 24 h for VDAC oligomerization by using EGS-based cross-linking and immunoblotting, as described for panel A. The relative amounts of dimers are given (RU, relative units), and the positions of VDAC monomers to tetramers and multimers are indicated. (D) Apoptosis, induced by UV irradiation, was assayed using annexin V-FITC-PI staining and FACS analysis. Results from one experiment representative of two similar experiments are shown.