FIG. 7.

Correlation between the extent of apoptosis and the level of VDAC oligomerization. (A) Immunoblot analysis shows the levels of VDAC oligomerization (cross-linking with 300 μM EGS for 15 min) induced by STS at various concentrations (0.1 to 0.6 μM for 16 h or 1.25 μM for 2.5 h) in T-REx-293 cells. (B) Quantitative analysis of the immunostained VDAC dimers and trimers and the extent of apoptosis, presented as a function of the STS concentration. (C to F) HeLa cells were exposed to H₂O₂ (12 mM) for the indicated times, after which VDAC oligomerization (C), apoptotic cell death (D), and Cyto c release (E) were analyzed and presented as a function of time (F).