Skip to main content
. 2010 Oct 11;30(24):5672–5685. doi: 10.1128/MCB.00878-10

FIG. 8.

FIG. 8.

UBA is necessary but JUBA is dispensable for Nbr1 function. All error bars represent SEM. P values were calculated by a one-tailed paired t test (n = 4). (A) Deletion of UBA but not JUBA abrogates Nbr1-mediated inhibition of EGFR degradation. HEK293T cells were transfected with Myc-Nbr1, Myc-Nbr1ΔUBA, Myc-Nbr1ΔJUBA, or GFP as a control. Cells were serum starved and stimulated with 50 ng/ml EGF for the indicated times before being lysed and analyzed by Western blotting. (B) Deletion of UBA but not JUBA abrogates Nbr1-mediated inhibition of FGFR2 degradation. HEK293T cells were transfected with Myc-Nbr1, Myc-Nbr1ΔUBA, Myc-Nbr1ΔJUBA, or GFP as a control. Cells were serum starved and stimulated with 20 ng/ml FGF2 plus 10 μg/ml heparin for the indicated times before being lysed and analyzed by Western blotting. (C) Densitometry analysis of RTK degradation by Myc-Nbr1, Myc-Nbr1ΔUBA, and Myc-Nbr1ΔJUBA from panels A and B.