Late endocytic localization of Nbr1 is independent of LIR. Scale bars, 10 μm. (A) A truncated Nbr1 mutation lacking LIR2 (Nbr1G694>) colocalizes with LAMP2. COS7 cells transfected with GFP-Nbr1G694> were fixed, immunostained for LAMP2, and analyzed by confocal microscopy. (B) This truncated mutation with an additional point mutation in the main LIR (Nbr1G694>Y750A) can still colocalize with LAMP2. COS7 cells transfected with GFP-Nbr1G694>Y750A were fixed, immunostained for LAMP2, and analyzed by confocal microscopy.