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. 2010 Oct 18;30(24):5710–5725. doi: 10.1128/MCB.00665-10

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FIG. 6. — MURF-3 mRNA and protein are regulated by IMP-2 in C2C12 myoblasts. (A and B) MURF-3 mRNA (A) and protein (B) are downregulated in IMP-2 KD C2C12 myoblasts (obtained with two distinct siRNAs [1 and 2]) and upregulated in two distinct stable C2C12 lines expressing FLAG-HA-IMP-2, tagged on the N terminus (siRNA 1) or C terminus (siRNA 2). (C) In IMP-2 KD C2C12 myoblasts (obtained with three distinct siRNAs [1, 2, and 3]), downregulation of MURF-3 is followed by a specific decrease of Glu-tubulin. The indicated mRNA levels of IMP-2 and MURF-3, expressed as a percentage of the control (transfection with control siRNA), were quantified by qRT-PCR and normalized by 36b4. (D and E) MURF-3 KD myoblasts and RMS cells show decreased motility (wound test [D]) and migration capacity (Transwell test [E]). Shown are the means of at least three independent experiments; error bars indicate standard deviation.