Skip to main content
. 2010 Oct 18;30(24):5710–5725. doi: 10.1128/MCB.00665-10

FIG. 8.

FIG. 8.

Recombinant IMP-2 directly binds the mRNAs of MURF-3 and PINCH-2. (A and B) RNA bandshift assays using increasing concentrations of GST-IMP-2 map the binding sites of IMP-2 in MURF-3 and PINCH-2 transcripts. The results for each RNA fragment were reproduced at least twice; shown is a typical result. nt, nucleotides. (C) Binding of recombinant IMP-2 to a fragment of H19, a previously characterized RNA target of IMPs. (D) GST-IMP-2 and GST proteins used in control experiments. (E and F) Specific binding of IMP-2 to the 5′ UTR of MURF-3 and 3′ UTR of PINCH-3 is confirmed by control experiments with GST and cold-probe competition.