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. 2010 Oct 18;30(24):5710–5725. doi: 10.1128/MCB.00665-10

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FIG. 9. — IMP-2 and its protein partners form distinct multiprotein-RNA complexes in myoblasts. (A) Silver staining of FLAG-HA-IMP-2 protein complexes purified from C2C12 myoblasts (lanes 2 and 3) proliferating (prol.) or differentiating (diff.) for 24 h. Mock, FLAG-HA immunoprecipitate (IP) from control C2C12 cells (lane 1). Lane 4, RNase A-treated IMP complexes from differentiating C2C12 cells. (B) Subcellular localization of IMP-2 and control FLAG-HA-tagged proteins stably expressed in C2C12 myoblasts; (C) expression levels of proteins shown in panel B; (D and E) FLAG-HA-IMP-2 complexes compared to irrelevant FLAG-HA complexes purified from C2C12 myoblasts shown in panels B and C; (D) silver staining; (E) Western blot against selected IMP-2 partners. (F) HA-FLAG-IMP-2 complexes were immunity purified from C2C12 myoblasts, separated on glycerol gradient, and analyzed by silver staining. RNAs were purified from each fraction, and the presence of the indicated IMP-2 RNA targets was quantified in each fraction by qRT-PCR.