FIG. 2.
Analysis of cytokines and metabolic parameters. Wild-type (WT) and p53S18A (S18A) mice were maintained on a standard chow diet. Experiments were performed on 6- to 7-month-old animals. (A to C) WT and p53S18A mice were fasted overnight, and the concentrations of TNF-α, IL-6, and IFN-γ in blood were measured (means ± SEM; n = 6 animals). (D) Insulin measurement in mice fasted overnight (means ± SEM; n = 9). (E) Body weight was measured at 6 months (means ± SEM; n = 15). (F) Concentration of triglyceride in the blood of WT and p53S18A mice was measured (means ± standard deviation [SD]; n = 3). (G to I) The concentrations of the adipokines leptin, adiponectin, and resistin in the blood of mice fasted overnight were measured (means ± SEM; n = 7). Statistically significant differences between WT and p53S18A mice are indicated (*, P < 0.05).