FIG. 4.

p53^S18A mice exhibit hepatic insulin resistance. (A and B) Wild-type (WT) and p53^S18A (S18A) mice were maintained on a standard chow diet. The data presented are the means ± SEM (n = 7 or 8). Statistically significant differences are indicated (*, P < 0.05). (A) Whole-body glycogen synthesis; (B) basal hepatic glucose production (HGP) (left) and insulin-stimulated HGP during the hyperinsulinemia-euglycemic clamp analysis (right). (C) Extracts prepared from the liver of WT and p53^S18A mice were examined by immunoblot analysis using antibodies to Akt and pSer473 Akt. The mice were fasted overnight and treated without and with insulin (1.5 U/kg body mass) by intraperitoneal injection (30 min).