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. Author manuscript; available in PMC: 2010 Dec 20.
Published in final edited form as: Nat Protoc. 2006;1(2):729–748. doi: 10.1038/nprot.2006.98
Component Amount (per reaction) Final amount/concentration
10× NEB buffer 2 11.0 μl 1×
1 μg μl−1 BSA 5.5 μl 50 μg ml−1
2.5 mM dNTP mix 4.4 μl 100 μ M each dNTP
T4 DNA polymerase (3 U μl−1) 0.5 μl 1.5 U
ddH2O 33.6 μl
TOTAL volume 55.0 μl