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. Author manuscript; available in PMC: 2010 Dec 20.
Published in final edited form as: Nat Protoc. 2006;1(2):729–748. doi: 10.1038/nprot.2006.98
Component Amount (per reaction) Final amount/concentration
5× T4 DNA ligase buffer 10.0 μl 1×
15 μM linker 6.7 μl 2 μM
T4 DNA ligase (400 U μl−1) 0.5 μl 200 U
ddH2O 7.8 μl
TOTAL volume 25.0 μl