Chromatin immunoprecipitation and microarray-based analysis of protein location

. Author manuscript; available in PMC: 2010 Dec 20.

Published in final edited form as: Nat Protoc. 2006;1(2):729–748. doi: 10.1038/nprot.2006.98

Cycle	Denature	Anneal	Extend
1	55 °C, 4 min	72 °C, 3 min
2	95 °C, 2 min
3–27	95 °C, 30 s	60 °C, 30 s	72 °C, 1 min
28	72 °C, 5 min