FIG. 5.
MyD88 is more critical in the adaptive immune response than TRIF. Groups of mice were infected with 5 × 102 PFU of PR8 virus, and samples were taken 4 weeks after infection. T-cell proliferation (A), Th1/Th2 cytokine profiles after in vitro culture (B), and virus antigen-specific CD8 T cells (C) were determined as described in the legend of Fig. 2. (D) IgG levels of serum and BALF and IgA levels of serum, BALF, nasal wash (NW), and saliva were determined by ELISA. Results are representative of at least two individual experiments (n ≥ 3 for each group). Data are means + standard deviations. *, P < 0.05; **, P < 0.01; and ***, P < 0.001 (compared with wild-type controls).