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Phylogenetic tree showing the relationship between Env variant sequences (V1 to V5) in macaque CA28. A neighbor-joining tree rooted on four functional sequences of the inoculating virus SHIVSF162P3N was generated. The scale bar indicates the genetic distance along the branches in nucleotides, and the asterisk at the node represents a bootstrap value of >70%. ⧫, representative clones in the inoculum; ▴, WT variants; •, RRW/RRW.A-bearing variants; ○, Δ22-25-bearing variants.
