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. 2010 Oct 13;84(24):12564–12575. doi: 10.1128/JVI.01559-10

FIG. 2.

FIG. 2.

Effect of Treg cell transfer on the course of IAV infection of lymphocyte-deficient mice. (A) Flow-cytometric detection of transferred Treg cells in the lungs of PR8-infected Rag1−/− mice (PR8 + Treg). As a control, the lungs of PR8-infected Rag1−/− mice that did not receive Treg cells (PR8) also were analyzed. Numbers within the plots represent the mean percentage of CD4+ TCRβ+ T cells in 5 to 9 mice per group analyzed 20 to 25 days after PR8 infection. (B) Percentage of Foxp3+ cells in CD4+ TCRβ+ T cells isolated from the spleen or the lungs of PR8-infected Rag1−/− recipients of Treg cell transfer. Numbers within the plots represent the mean percentages of Foxp3+ cells in six mice analyzed 25 days after PR8 infection. (C) Weight change (left) and survival (right) of PR8-infected Rag1−/− mice in the presence (PR8 + Treg) or absence (PR8) of adoptively transferred Treg cells. Data are from 12 mice analyzed in three separate experiments. Median survival times were 18.0 days for PR8 and 22.5 days for PR8 + Treg (P = 0.004). (D) Levels of PR8 matrix mRNA over time in the lungs of PR8-infected Rag1−/− mice with (PR8 + Treg) or without (PR8) adoptively transferred Treg cells. Each symbol represents an individual mouse.