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Propagation kinetics of fluorescent viruses. Single-step growth curves of unmodified HSV-1 strain F (wild type) and three recombinant derivatives (RFP-cap, GFP-env, and RFP-cap/GFP-env) are shown. Infected cell supernatants (dashed lines, open symbols) and adherent Vero cells (solid lines, filled symbols) were harvested at the indicated times, and titers were quantitated by plaque assay.
