FIG. 5.

Capsids are transported with gB during egress in axons. Dissociated DRG sensory neurons from chick (A) or rat (C) or a differentiated mouse CNS cell line (CAD) (D) were infected with HSV-1 RFP-cap/GFP-env, and axons were imaged following replication from 18 to 24 h postinfection. Montages are frames of alternating RFP-capsid (R) and GFP-gB (G) emissions captured with continuous 100-ms exposures. Each montage documents the motion of a single viral particle toward the axon terminal. Frames are 2.5 μm by 20.5 μm (A), 2.5 μm by 21 μm (C), or 2.5 μm by 11 μm (D). (B) The numbers of RFP-capsids that are transported with (gray) or without (black) GFP-gB in chick DRG sensory neurons. (E) Percentages of anterograde moving capsids that are cotransported with GFP-gB in different neuronal cell types (left) and the percentage of GFP-gB incorporation into extracellular viral particles as derived from Fig. 3 (right). n, number of capsids tracked.