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. 2010 Sep 1;84(24):13019–13030. doi: 10.1128/JVI.01296-10

FIG. 7.

FIG. 7.

Anti-VP5 antibodies are partially excluded from labeling intact virions but not unenveloped capsids. (A and B) Vero cell (A) or dissociated chick DRG sensory neuron (B) fixed at 24 h postinfection with HSV-1 RFP-cap (red) and stained with a combination of two antibodies directed against VP5 (green). VP5 antibodies labeled capsids present in the nucleus but displayed poor staining of RFP-capsids present in the cytoplasm (A) or axon (B). Frames are 30 μm by 30 μm (A) or 31.5 μm by 10.5 μm (B). (C) Imaging of individual viral nucleocapsids isolated from HSV-1 RFP-cap-infected nuclei (left panel), extracellular virions purified from HSV-1 RFP-cap-infected Vero cell supernatants (middle panel), or viral particles released from Vero cells onto coverslips at 2 to 3 days postinfection with HSV-1 RFP-cap (right panel) are shown. Nucleocapsids and purified virions were seeded on coverslips for 2 h before fixation and were stained with either a combination of two VP5-specific antibodies or an antibody directed against gC. Examples of mRFP1-VP26 (red-capsid) and anti-VP5 or anti-gC (green) emissions from the same 24-μm by 24-μm field are shown. Graphs below each panel represent the fraction of mRFP1 particles that emit green antibody fluorescence (n, number of RFP-capsids).