FIG. 2.

Amino acid residues near the C-terminal end of PIV5 NP protein are important for VLP production. (A) Comparison of paramyxovirus NP/N protein C-terminal amino acid sequences. Sequence identities with PIV5 that are within 5 amino acid residues of the C-terminal end are shown in boldface. (B) Schematic illustrating NP protein amino acid substitutions. (C) 293T cells were transfected to produce PIV5 M and HN proteins together with the indicated NP protein variants, and VLP production was measured as described in the legend to Fig. 1. (D) Relative VLP production efficiency values were calculated from three independent experiments performed as indicated for panel C; error bars indicate standard deviations.