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Second-site mutations to M protein restore interaction with NP 508A protein. 293T cells were transfected to produce the indicated NP protein variants together with M protein harboring the I278M second-site mutation (A) or M protein harboring the G318D second-site mutation (B). Detergent-free cell extracts were prepared by Dounce homogenization, and coflotation analysis was performed as described in the legend to Fig. 3. The data shown are representative of two independent experiments.
