TABLE 1.

Oligonucleotides used to generate mutated U1 snRNAs

Oligonucleotide	Sequence
U1D2upEx-F	5′CTCCCCTGCCAGGTGAAGGTGAGATCTTCGG3′
U1D2upEx-R	5′CCGAAGATCTCACCTTCACCTGGCAGGGGAG3′
U1D3up-F	5′CTCCCCTGCCAGGTAGGAATGAGATCTTCGG3′
U1D3Up-R	5′CCGAAGATCTCATTCCTACCTGGCAGGGGAG3′
U1D2up-F	5′CTCCCCTGCCAGGTAAAGATGAGATCTTCGG3′
U1D2up-R	5′CCGAAGATCTCATCTTTACCTGGCAGGGGAG3′
UST1	5′GGGCGAAGATCTCATTCTAGTCTAGCAGGGGAGATACCATGATCACGAACGG3′
UAS	5′GGGTCAGCACATCCGGAGTG3′
U1T2-F	5′CCACCTTCGTGATCATGGTATCTCCCCTGAGCCAGTAGAATGAGATCTTCGGGCTCTGCCCCGACACAGCC3′
U1T2-R	5′GGCTGTGTCGGGGCAGAGCCCGAAGATCTCATTCTACTGGCTCAGGGGAGATACCATGATCACGAAGGTGG3′
U1T3-F	5′CCACCTTCGTGATCATGGTATCTCCCCTGGGAAGCATCCATGAGATCTTCGGGCTCTGCCCCGACACAGCC3′
U1T3-R	5′GGCTGTGTCGGGGCAGAGCCCGAAGATCTCATGGATGCTTCCCAGGGGAGATACCATGATCACGAAGGTGG3′
U1T4-F	5′CCACCTTCGTGATCATGGTATCTCCCCTGGTCAGCCTAAATGAGATCTTCGGGCTCTGCCCCGACACAGCC3′
U1T4-R	5′GGCTGTGTCGGGGCAGAGCCCGAAGATCTCATTTAGGCTGACCAGGGGAGATACCATGATCACGAAGGTGG3′
U1T5-F	5′CCACCTTCGTGATCATGGTATCTCCCCTGCAGAGGAGAGATGAGATCTTCGGGCTCTGCCCCGACACAGCC3′
U1T5-R	5′GGCTGTGTCGGGCAGAGAGCCCGAAGATCTCATCTCTCCTCTGCAGGGGAGATACCATGATCACGAAGGTGG3′
U1T6-F	5′CCACCTTCGTGATCATGGTATCTCCCCTGACTGCTTGTAATGAGATCTTCGGGCTCTGCCCCGACACAGCC3′
U1T6-R	5′GGCTGTCGGGGCAGAGCCCGAAGATCTCATTACAAGCAGTCAGGGGAGATACCATGATCACGAAGGTGG3′