TABLE 1.

Gene-specific primers used for constructing recombinant plasmids

^aFWPV, egfp, and dsRed gene-specific forward (FP) and reverse (RP) primers were designed based on sequences of the FWPV genome, pEGFP-C1 (Clontech), and pDsRed-Monomer-C1 (Clontech), respectively.

^bRestriction sites are as follows: NheI (single underlining), NsiI (single dotted line), XmaI (double underlining), SacII (wavy line), KpnI (boxed), and NotI (light shading). Also indicated are a Myc tag coding sequence (lowercase roman), the synthetic vaccinia virus early/late promoter (dark shading), the complement of sequence encoding the C terminus of the egfp gene (double wavy line) plus the N terminus (bold), and the C terminus (lowercase italics) of the dsRed.