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. 2010 Oct 6;84(24):12914–12923. doi: 10.1128/JVI.00864-10

FIG. 1.

FIG. 1.

RDV Pns10 suppressor activity in transgenic N. benthamiana 16c/Pns10, in which GFP and Pns10 were stably expressed. (A) Western blot of total proteins extracted from 16c and 16c/Pns10 plant leaves, probed with polyclonal antisera specific for Pns10. Coomassie blue-stained Rubisco proteins were used as the loading control. (B) N. benthamiana 16c and 16c/Pns10 plants were infiltrated with Agrobacterium carrying 35S-GFP. GFP fluorescence was imaged under long-wavelength UV light at 5 dpi. (C) GFP fluorescence was monitored for the onset of systemic silencing at 28 dpi. Of 12 infiltrated 16c/Pns10 plants, 11 showed GFP fluorescence indicative of suppression of silencing by Pns10. All 13 infiltrated 16c plants, as controls, lacked GFP fluorescence, a finding indicative of GFP silencing. (D) Northern blots showing the steady-state levels of GFP mRNAs and siRNAs extracted from the different infiltrated patches shown in panels B and C. The bottom panel shows rRNA or tRNA stained with ethidium bromide as a loading control.