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. 2010 Oct 6;84(24):12914–12923. doi: 10.1128/JVI.00864-10

FIG. 2.

FIG. 2.

Pns10-enhanced PVX-GFP infection and spread in N. benthamiana Nb/Pns10 plant lines. (A) PVX-GFP-infected nontransgenic N. benthamiana (Nb) and Nb/Pns10 plants under UV light at 5, 7, 15, and 28 dpi. The inoculated leaves (inoc) and successive systemic leaves (t1 to t3) are indicated. (B) Northern blots of PVX-GFP RNA accumulations in inoculated leaves of N. benthamiana (Nb) and Nb/Pns10 plants at 3 and 7 dpi. The membranes were hybridized with DIG-labeled probes corresponding to the full-length ORF of PVX CP. (C) Northern blot of PVX-GFP RNA in top systemic leaves of N. benthamiana (Nb) and Nb/Pns10 plants at 5, 15, and 28 dpi.