Skip to main content
. 2010 Oct 13;84(24):13059–13062. doi: 10.1128/JVI.00912-10

FIG. 1.

FIG. 1.

Cell type-specific expression of B19V capsid proteins. (A) Relevant positions of B19V genes and promoter (p6) in B19V genome. (B) Immunoblotting of B19V capsid protein production in various cells. CD36+ EPCs and UT7/Epo-S1 cells were transfected using the Amaxa Cell Line Nucleofector kit, and 293T and HeLa cells were transfected using Lipofectamine 2000. Whole-cell lysates were prepared at 48 hpt, resolved on 4 to 20% SDS-PAGE gels, and subjected to immunoblotting with anti-Flag antibody. Bands were visualized by using SuperSignal chemiluminescent reagent and then exposure to X-ray film. Numbers on the left indicate the molecular masses (kDa). GAPDH, glyceraldehyde-3-phosphate dehydrogenase.