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. 2010 Oct 6;84(24):12824–12831. doi: 10.1128/JVI.01487-10

FIG. 2.

FIG. 2.

Analysis of 200-nt deletions in DHBV. (A) Representation of 200-nt deletion variants. Nucleotide coordinates refer to the specific sequence deleted or substituted in the WT pgRNA. Oligonucleotide 2b anneals near the 5′ end of minus-strand DNA and produces an extension product of approximately 79 nt. cis sequences necessary for encapsidation (i.e., ɛ and region II) were not analyzed. White boxes, DR sequences. Black box, region II. (B) Primer extension analysis of variants. Products representing the 5′ ends of pgRNA and minus-strand DNA are indicated. Relative levels of minus-strand DNA were calculated as described in text.