FIG. 7.

Expression and virion incorporation of M* protein. (A) Western blots of lysates from 17Cl1 cells infected at a multiplicity of 0.1 PFU per cell with wild-type MHV or with each of the mutants shown in Fig. 6A and harvested at 16 h postinfection. The blots were probed with anti-M monoclonal antibody J.1.3 (α-M) (top) or anti-E polyclonal antibody (α-E) (bottom). mock, control lysate from mock-infected cells. (B) Western blots of purified wild-type or M*/ΔE mutant virions probed with anti-E polyclonal antibody (left), anti-M monoclonal antibody J.1.3 (middle), or anti-N monoclonal antibody J.3.3 (α-N) (right). Molecular mass standards (kDa) are indicated on the left of each panel.