FIG. 6.

Subcellular localization of core protein. (A) Huh-7 cells were electroporated with in vitro transcribed HCV genomes containing the indicated mutations. Cells were fixed at 72 h postelectroporation, and core protein was detected with MAb anti-core 2H9 (red). Lipid droplets were stained with BODIPY 493/503 (green). (B) Fixed cells at 72 h postelectroporation were double labeled with MAb anti-core (2H9/IgG1) (green) and MAb anti-NS5A (9E10/IgG2a) (red). For both panels, merged images and zoomed views of the indicated areas are shown. Nuclei were stained with DAPI (blue). Representative confocal images are shown. Bar, 10 μm.