FIG. 1.

Schematic of FHV genome components and the corresponding expression cassettes used in Drosophila expression plasmids. Each FHV component is expressed by a baculovirus IE1 promoter in a plasmid that also contains the baculovirus transactivating hr5 enhancer. Plasmid pF1 expresses RNA1, and pF2 expresses RNA2 with a δRz-mediated authentic 3′ end (δRz). pF1 and pF2 contain 46 nonviral nt between the IE1 transcription start site and the first viral nucleotide. pFA encodes protein A mRNA with nonviral 5′ and 3′ untranslated sequences and a 3′ polyadenylation signal (An). pFA contains 52 nonviral nt between the IE1 transcription start site and the initiating methionine codon.