FIG. 1.

Distribution of TMPRSS2 and ACE2 and histopathology of SARS-CoV-infected cynomolgus lung. The distribution of TMPRSS2 (A, arrows indicating round shapes in middle panel) and ACE2 (C, arrows indicating thin shapes in middle panel) in healthy cynomolgus lung sections stained with antibodies against SARS-CoV, TMPRSS2, or DAPI (upper panels) was detected by an immunofluorescence staining method (see Materials and Methods section). Samples stained with secondary antibodies were used as controls (lower panels). The distribution of TMPRSS2 (B) and ACE2 (D) was also examined in mild (upper row) and severe (lower row) lesions of SARS-CoV-infected lungs stained with antibodies against SARS-CoV, TMPRSS2, or DAPI (nuclear staining).