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Effect of TMPRSS2 on virus entry into cells. Vero or Vero-TMPRSS2 cells were infected with SARS-CoV at an MOI of 0.1 in the presence of 5 μM HR2 peptide, 50 μM EST, or 500 μM bafilomycin A1 and then cultured for 5 h. The amount of viral mRNA9 was measured by real-time PCR. Cells not treated with reagents were used as controls. The error bars are standard deviations of at least six independent measurements.
