FIG. 6.
Role of amphipathic α-helix AH2 in NS4B oligomerization. (A) Constructs pCMVNS4B-CFP and pCMVNS4B-YFP (both designated wt) as well as pCMVNS4BAH2mut-YFP and pCMVNS4BAH2mut-CFP (both designated AH2mut) were transfected into U-2 OS cells in different combinations, as indicated. (B) Constructs pCMVNS4B40-130-CFP and pCMVNS4B40-130-YFP (both designated wt), pCMVNS4B40-130AH2mut-YFP and pCMVNS4B40-130AH2mut-CFP (both designated AH2mut), and pCMVNS4B130-261-YFP (130-261) were transfected into U-2 OS cells in different combinations, as indicated. The significance of the observed differences was assessed as described in Materials and Methods (*, P < 0.0001; #, P = 0.0036).