Inhibition of HEV Rluc replicon replication by LNAs. (A) The position of LNA on the stem-loop structure in the junction region of the HEV genome (targeting nt 5105 through 5126) is indicated with a boldface solid line. (B) Relative luciferase activity in Huh7 S10-3 cells cotransfected at the same time with HEV Rluc replicon and LNA sense, LNA antisense, or control oligonucleotide (100 pmol per well in 24-well plate), respectively. (C) Relative luciferase activity in Huh7 S10-3 cells transfected with LNA sense, LNA antisense, or control oligonucleotide at 48 h after the transfection of the HEV Rluc replicon RNA. Data are from an average of eight separate replicate experiments, and the error bars indicate SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001.