TABLE 1.
Primers used in the generation of HEV mutants and LNAs used for inhibition of HEV replicon replication
| Oligonucleotide | Polaritya | nt positionb | Sequencec |
|---|---|---|---|
| Mutagenic primers | |||
| HEV2m1R | − | 5090-5139 | 5′-CGAACCCATGGGCGCAGCAAATGACATGTTATTCATTCCACCCGACACAG-3′ |
| HEV2m1F | + | 5′-CTGTGTCGGGTGGAATGAATAACATGTCATTTGCTGCGCCCATGGGTTCG-3′ | |
| HEV2m2R | − | 5097-5141 | 5′-CGCGAACCCATGGGCGCATTAAAAGACATGTTATTCATTCCACCC-3′ |
| HEV2m2F | + | 5′-GGGTGGAATGAATAACATGTCTTTTAATGCGCCCATGGGTTCGCG-3′ | |
| HEV2m3R | − | 5094-5140 | 5′-GCGAACCCATGGGCGCAGCAAATCTCATGTTATTCATTCCACCCGAC-3′ |
| HEV2m3F | + | 5′-GTCGGGTGGAATGAATAACATGAGATTTGCTGCGCCCATGGGTTCGC-3′ | |
| HEV2m4R | − | 5094-5140 | 5′-GCGAACCCATGGGCGCAGCATTTCTCATGTTATTCATTCCACCCGAC-3′ |
| HEV2m4F | + | 5′-GTCGGGTGGAATGAATAACATGAGAAATGCTGCGCCCATGGGTTCGC-3′ | |
| HEV2m5R | − | 5085-5134 | 5′-CCATGGGCGCAGCAAAAGACAACAAATTCATTCCACCCGACACAGAATTG-3′ |
| HEV2m5F | + | 5′-CAATTCTGTGTCGGGTGGAATGAATTTGTTGTCTTTTGCTGCGCCCATGG-3′ | |
| HEV2m6R | − | 5090-5139 | 5′-CGAACCCATGGGCGCAGCAATTGACATGTTATTCATTCCACCCGACACAG-3′ |
| HEV2m6F | + | 5′-CTGTGTCGGGTGGAATGAATAACATGTCAATTGCTGCGCCCATGGGTTCG-3′ | |
| HEV2m7R | − | 5079-5125 | 5′-CAGCAAAAGACATGTTATTCATTCGACCCGACACAGAATTGAATTTG-3′ |
| HEV2m7F | + | 5′-CAAATTCAATTCTGTGTCGGGTCGAATGAATAACATGTCTTTTGCTG-3′ | |
| HEV2m8R | − | 5101-5144 | 5′-GGTCGCGAACCCATGGGCGATGCAAAAGACATGTTATTCATTCC-3′ |
| HEV2m8F | + | 5′-GGAATGAATAACATGTCTTTTGCATCGCCCATGGGTTCGCGACC-3′ | |
| HEV2m9L | − | 5090-5143 | 5′-GGTCGCGAACCCATGGGCGCTTGTAAAGACATGTTATTCATTCCACCCGACACAG-3′ |
| HEV2m9U | + | 5′-CTGTGTCGGGTGGAATGAATAACATGTCTTTACAAGCGCCCATGGGTTCGCGAC-3′ | |
| HEV2m59L | − | 5090-5144 | 5′-GGTCGCGAACCCATGGGCGCTTGTAAAGACAACAAATTCATTCCACCCGACACAG-3′ |
| HEV2m59U | + | 5′-CTGTGTCGGGTGGAATGAATTTGTTGTCTTTACAAGCGCCCATGGGTTCGCGACC-3′ | |
| HEV2mGAAU | + | 4655-4705 | 5′-CAGGTGGCTGCCTTTAAAGGTGCCGCCTCGATAGTGCTTTGCAGTGAGTAC-3′ |
| HEV2mGAAL | − | 5′-GTACTCACTGCAAAGCACTATCGAGGCGGCACCTTTAAAGGCAGCCACCTG-3′ | |
| LNAs | |||
| p19 antisense | − | 5105-5126 | 5′-G+CAG+CA+AA+AG+AC+ATGTT+ATT+CA-3′ |
| p19 sense | + | 5′-T+GAA+TAACA+TGT+CTTT+TG+CT+GC-3′ |
a
Polarity of primers or LNAs on the HEV genome. +, forward; −, reverse.
b
Positions of primers or LNAs on the HEV genome.
c
Sequences of primers or LNAs. The mutated nucleotides are underlined and in boldface. The modified nucleotides in LNAs are indicated with +.