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Growth of the recombinant viruses in cell culture. MDCK (A) and A549 (B) cells in six well plates were infected in triplicates with the viruses at MOIs of 0.001 and 0.01, respectively. At the indicated time points, supernatants were harvested and stored at −70°C. Virus titers were determined on MDCK cells. Error bars indicate standard deviations.
