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. Author manuscript; available in PMC: 2010 Dec 20.
Published in final edited form as: Mol Cell. 2004 Oct 22;16(2):199–209. doi: 10.1016/j.molcel.2004.09.021

Figure 2.

Figure 2

Recruitment of Hst1 to Promoters of Sporulation and Kynureine Genes by the Sum1 Repressor

(A) The binding ratio for Hst1 (blue), Sum1 (red), and Hst1 in sum1Δ cells (green) across the SPS4 and BNA5 loci, as determined by ChIP, is shown. A diagram of the regions amplified by sequence-specific PCR is shown on top.

(B) The log binding ratio (as determined by genome-wide location analysis) of Hst1, Sum1, and Rpd3 (used as a control) is displayed for the promoters of genes bound by Hst1 (p < 0.005). The log binding ratio of Hst1 in sum1 deletion cells (sum1Δ) is also shown.

(C) The level of histone H3 (K9/K14) acetylation and histone H4 (K5, K8, K12, K16) acetylation, as determined by ChIP, is shown in wild-type cells (black) and in cells deleted for HST1 (gray) at the SPS4 and BNA5 promoters. The binding ratios in (A) and (C) were all calculated as in Figure 1B. The binding ratios in hst1Δ in (C) were normalized to wt. These experiments were repeated multiple times and the variation was never more than 15%.