Figure 4.

Representative flow cytometric histograms of the induced apoptosis of CD11b⁺ monocytes treated with Pc 4-PDT. Total blood was obtained from patients diagnosed with Sezary syndrome. The histograms shown were taken from a single patient with SS. PBMCs were isolated, treated with Pc 4, and stained as mentioned previously in the Methods section. Monocytes were identified by their high CD11b⁺ expression (y-axis) and forward scatter (x-axis) as shown in yellow (Region R18) in (a). Levels of apoptosis in all three Pc 4-PDT doses were then further analyzed from this population. As shown in (c), (d), and (e), levels of apoptosis were similar in the three treatment groups. Apoptosis induction in the untreated group is shown in (f).