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PBMC proliferative responses to NS3358–375 peptide variants and mixes with wild-type peptide in cultures depleted of CD4+CD25+ Treg cells. Subject 3019 total or Treg-depleted PBMC were stimulated with peptide variants and measured 3H-TdR-uptake. Results are mean counts of six wells each +/- SEM, representative of three independent experiments. Gray bar, individual peptide; stripped bars, CD4+CD25+ Treg-depleted PMBC. *P < .05 as determined by Student's t-test. **P < .005.
