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DRB1*1501 MHC class II variant and cognate tetramers are able to bind to CD4+ T-cells from multiple patients. PBMCs from a noninfected (P1163), chronic (P.B3019), and resolved (JVP008) were individually stained with DR15 MHC class II tetramers (2 μg/ml). P.1163 is a noninfected DR15 subject and was used as a control to test for nonspecific labeling of each DR15 tetramer (first column). DR15-CLIP-APC and -PE tetramers were used as a control for each experiment (bottom two rows).
