Table 2.
CD4+ and CD8+ T cell responses to rNS3 and H3 Ags as shown as frequency of CFSElow and the transformation of T cell proliferation data. Frequency of CD4+ and CD8+ T cells in CFSE-labeled lymphocytes when stimulated with rNS3 and H3 at 1 μg/ml for 7 days and analyzed by flow cytometry. On Day 7, the cells were stained with CD4 and CD8. The proliferative response was determined by the medium for each subject. log10Δmax is the transformation of the T cell prolfieration data for each subject and the respective antigen stimulation.
| Subject | Medium | NS3 | H3 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| CD4 | CD8 | CD4 | CD8 | log10Δmax | CD4 | CD8 | log10Δmax | ||
| RLM 037 | R | 0.97 | 1.49 | 6.97 | 7.1 | 4.4 | 1.49 | 4.91 | 0 |
| KML044 | R | 0.29 | 0.53 | 4.06 | 10.7 | 4 | 0.27 | 0.63 | 0 |
| ZSS035 | R | 0.6 | 0.95 | 4.12 | 2.65 | 4.2 | 3.73 | 1.85 | 4.1 |
| LEC028 | R | 1.16 | 0.54 | 6 | 2.4 | 4 | 1.81 | 0.8 | 2 |
| KTJ010 | R | 1.33 | 1.32 | 4.98 | 2.92 | 4 | 1.88 | 1.95 | 2.5 |
| BPB026 | R | 0.4 | 0.29 | 1.58 | 1.12 | 3.2 | 0.42 | 0.22 | 2.7 |
| DRB012 | R | 1.22 | 0.77 | 0.8 | 3.68 | 2.5 | 0.8 | 3.68 | 3.5 |
| JVP008 | R | 0.4 | 0.46 | 2.61 | 4.07 | 3.2 | 0.8 | 0.47 | 3 |
| JPZ061 | R | 1.34 | 1.76 | 1.1 | 1.41 | 3.3 | 1.15 | 1.3 | 0 |
| AJG066 | C | 4.31 | 4.84 | 4.73 | 4.8 | 3.4 | 4.81 | 4.68 | 0 |
| KRW002 | C | 4.79 | 4.6 | 5.29 | 5.19 | 3.8 | 5.43 | 4.75 | 3.5 |
| MH065 | C | 6.31 | 7.21 | 6.97 | 7.29 | 3.6 | 6.6 | 7.3 | 3.2 |
| NLM049 | C | 1.84 | 4.47 | 2.01 | 14.2 | 3.5 | 0.18 | 0.84 | 3.05 |
| CER014 | C | 0.68 | 0.34 | 1.04 | 0.75 | 4 | 0.74 | 0.33 | 3 |
| SSB007 | C | 22.7 | 29.1 | 23.8 | 31.5 | 3.9 | 23.1 | 28.1 | 0 |
| RLW027 | C | 0.16 | 0.44 | 0.33 | 1.99 | 4.2 | 0.18 | 0.84 | 3.8 |