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Cytogram for TUNEL. (a, d) The negative control was incubated without the TdT enzyme. (b, e) Positive control was prepared before the labeling reaction with an additional treatment with DNAse I. (c, f) The TUNEL-positive cell in the fertile donor and patient sample was measured, respectively, with respect to the negative-control sperm population using a 530 nm ± 30 nm band-pass filter. A total of 10 000 events were measured at a flow rate of 200–300 cells/s on a flow cytometer.
