Figure 3. Tg induces aggregation of STIM2.

(A) Immunofluorescence labeling of DCs for (A) STIM1 and (B) STIM2 under control conditions (unstimulated) and after Tg treatment (Stimulated; 2 μM Tg for 10 min). Fixed cells were permeabilized and stained with STIM1 and STIM2 antibody followed by a secondary antibody (FITC-conjugated), which alone, produced negligible staining (see Supplemental Fig. 2B).