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Components of pri-miRNA/SC35-containing nuclear foci. Cells transfected with pri-let-7 were subjected to IF using antibodies directed against (A) ASF/SF2, (B) PABII, (C) Pol II or (E) PM/Scl-100, followed by in situ hybridization to pri-let-7. In (D), cells were cotransfected with pri-let-7 and GFP-Pin1, and ISH to pri-let-7 was performed. Arrowheads in (B) (part b) indicate untransfected cells. Scale bars, 10 μm.
