Figure 10.
O2·− levels were lower in NGF-deprived and -maintained SCG neurons lacking caspase 3. A, Genetic deletion of caspase 3 slightly, but significantly, reduced O2·− levels in NGF-supported casp3−/− SCG neurons. n = 234–559 neurons. B, Genetic reduction or deletion of caspase 3 significantly lowered O2·− levels in NGF-deprived casp3+/− and casp3−/− neurons. Cytochrome c was released over the same time course in the casp3−/− neurons as in wild type (data not shown). n = 182–559 neurons. MitoSOX dye intensities are normalized to the intensity averages of sibling wild-type cultures (with NGF from time of plating). *p < 0.01 compared with casp3+/+.