Figure 2.

EHD1-positive endosomal compartments in neurons are dynamic. A–C, Neurons were transfected with Cherry–EHD1. Images were captured with either 10, 1, or 0.5 s frame rates and total duration of 10 min, 60 s, and 30 s, respectively. The first movie frame of a part of dendrite is shown with the corresponding kymographs underneath. The time dimension is shown on the y-axis, and the retrograde direction is indicated above. Arrows indicate stationary vesicles and arrowheads motile vesicles. Scale bar, 6 μm. B′, C′, Trajectories of anterograde and retrograde moving vesicles from B and C. D–F, Examples of presumptive fusion (E, F) and budding (D, F) of Cherry–EHD1 vesicles during either 30 s or 10 min time lapse. G, Examples of dynamic shape changes of stationary vesicles in the soma, which include the temporary extensions of tubular domains (arrowheads; 3 s frame rate). H, Single frames of first time lapse from G magnified 2× are shown to illustrate example of tubular extension (arrowhead). I, Examples of changes in fluorescence intensity of Cherry–EHD1 vesicles during 10 min time lapse.