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. 2010 Dec 8;30(49):16485–16497. doi: 10.1523/JNEUROSCI.3127-10.2010

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Copyright © 2010 the authors 0270-6474/10/3016485-13$15.00/0

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Figure 4. — EHD1 localizes prominently to EEA1-positive early endosomes in neurons. A, Neurons were transfected with FLAG–EHD1 (red) and stained for endogenous EEA1 (cyan) (colocalization appears white; arrowheads). Single channels as well as overlaid channels are shown for the somatodendritic region and a part of dendrite. A′, Representative intensity line scans of FLAG–EHD1 and EEA1 are shown for part of a dendrite. Overlapping peaks for FLAG–EHD1 (red) and EEA1 (cyan) are visible. B, Neurons were transfected with FLAG–EHD1 and stained for endogenous NEEP21. B′, Representative intensity line scans of FLAG–EHD1 and NEEP21 are shown for a piece of dendrite. C, Neurons were cotransfected with FLAG–EHD1 and rab11–GFP. C′, Representative intensity line scans of FLAG–EHD1 and rab11–GFP are shown for a piece of dendrite. AU, Arbitrary units.