Table 2.

Fluorescence intensity changes of Cherry–EHD1 and GFP–EEA1 in endosomes

EHD1 and EEA1(n = 100)	EEA1 decreasing	EEA1 constant	EEA1 increasing
EHD1 decreasing	7	9	3
EHD1 constant	31	36	8
EHD1 increasing	1	2	3

Fluorescence intensity change of single vesicle was reported when fluorescence intensity of either Cherry–EHD1 or GFP–EEA1 or both markers at the end of the 1 and 10 min time lapse was equal or more than 16% of the fluorescence intensity at the beginning of the time lapse. Numbers of vesicles in which EEA1 fluorescence was either constant or decreasing or increasing are shown in columns. Numbers of vesicles in which EHD1 fluorescence was constant, decreasing, or increasing are shown in rows (n = 100).