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. 2010 Dec 20;5(12):e15188. doi: 10.1371/journal.pone.0015188

Figure 3. Glucocorticoid responsiveness of GBRs.

Figure 3

Reporter plasmids that harbor distinct GBRs were co-transfected with pcDNA3-hGR (150 ng) and pRL (100 ng) into 3T3-L1 preadipocytes in a 24-well plate (n = 6 per group). pRL plasmid provided Renilla luciferase expression to document transfection efficiency. Transfected cells were left overnight, then washed with PBS and treated with 0.5 µM DEX for an additional 16–20 hrs. Cells were then lysed and assayed for firefly and Renilla luciferase activities. Data shows fold-induction of luciferase activity (DEX/ethanol treated samples) from at least 6 experiments (*, p<0.05). The error bars represent the S. E. for the fold induction. The dashed-line represents one fold.